Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass

摘要

Background: To reduce the cost of the enzymes for the hydrolysis of lignocellulosic biomass, two main strategies\udhave been followed: one, the reduction of enzyme dosing by the use of more efficient and stable enzymatic cocktails;\udanother, to include accessory enzymes in the cocktails to increase yields by reducing the recalcitrant carbohydrate\udfraction remaining at the end of the process. To guide this second strategy, we have explored the chemical bond\udcomposition of different fractions of recalcitrant carbohydrates after enzymatic hydrolysis.\udResults: Two lignocellulosic feedstocks of relevance for the biofuels industry have been analyzed, corn stover and\udsugarcane straw. On comparing the composition of chemical bonds of the starting pretreated material with samples\udafter standard and forced hydrolysis (with enzyme overdosing), we obtained similar sugar and chemical bond\udcomposition.\udConclusions: This suggests that the current enzymatic cocktails bear the set of enzymes needed to hydrolyze these\udfeedstocks. From our point of view, the results show the need for a parallel fine-tuning of the enzymatic cocktails with\udthe pretreatment process to maximize sugar release yield